Biotechniques

Biotechniques

生物技术

  • 4区 中科院分区
  • Q3 JCR分区

高引用文章

文章名称 引用次数
Recent advances in photo-crosslinkable hydrogels for biomedical applications 36
Overlooked benefits of using polyclonal antibodies 12
Using synthetic oligonucleotides as standards in probe-based qPCR 8
A fast and simple fluorometric method to detect cell death in 3D intestinal organoids 7
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary 6
qPCR-based methods for expression analysis of miRNAs 6
Rapid quantification of cellular proliferation and migration using ImageJ 6
Protein extraction from human anagen head hairs one millimeter or less in total length 6
Guidelines for optimized gene knockout using CRISPR/Cas9 5
Chromium sequencing: the doors open for genomics of obligate plant pathogens 5
A gas trapping method for high-throughput metabolic experiments 4
Quantifying cell-free DNA in urine: comparison between commercial kits, impact of gender and inter-individual variation 4
A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging 4
Isolation of spheroid-forming single cells from gastric cancer cell lines: enrichment of cancer stem-like cells 4
Spike-in genomic DNA for validating performance of metagenomics workflows 4
A bioinformatics approach to identify telomere sequences 4
Use of FTA (R) classic cards for epigenetic analysis of sperm DNA 4
A triple co-culture method to investigate the effect of macrophages and fibroblasts on myoblast proliferation and migration 4
Removal of extra sequences with I-Scel in combination with CRISPR/Cas9 technique for precise gene editing in Drosophila 4
High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures 3
Rapid identification of genome-edited mesenchymal stem cell colonies via Cas9 3
Evaluation of miRNA detection methods for the analytical characteristics necessary for clinical utilization 3
Improved yield and accuracy for DNA extraction in microbiome studies with variation in microbial biomass 3
Forensic luminol reaction for detecting fecal occult blood in experimental mice 3
Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples 3
Detection of nucleic acids with a novel stem-loop primer rolling circle amplification technique 3
A robust fractionation method for protein subcellular localization studies in Escherichia coli 3
Clarification and confocal imaging of the nonhuman primate placental micro-anatomy 3
Development of a universal internal positive control 3
A novel, easy and rapid method for constructing yeast two-hybrid vectors using In-Fusion technology 3
Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening 3
Optimizing leading edge F-action labeling using multiple action probes, fixation methods and imaging modalities 3
Highly efficient library preparation for Ion Torrent sequencing using Y-adapters 3
Suitability of melanoma FFPE samples for NGS libraries: time and quality thresholds for downstream molecular tests 2
Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry 2
Idiosyncrasies of thermofluorimetric aptamer binding assays 2
Cell detachment rapidly induces changes in noncoding RNA expression in human mesenchymal stromal cells 2
An efficient isothermal PCR method for on-site detection of nucleic acid 2
The flash-small-pool PCR: how to transform blotting and numerous hybridization steps into a simple denatured PCR 2
Determination of polyurethane-grafted peptide (GSGREDVGSG) using bicinchoninic acid (BCA) assay 2
A modified gas-trapping method for high-throughput metabolic experiments in Drosophila melanogaster 2
Cataract-preventing contact lens for in vivo imaging of mouse retina 2
Capture and storage of plant genomic DNA on a readily available cellulose matrix 2
A PAGE screening approach for identifying CRISPR-Cas9-induced mutations in zebrafish 2
A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus 2
Immunochromatographic detection of MPB64 secreted from active BCG by heating: toward same-day diagnosis of tuberculosis 2
Bead-based assay for spatiotemporal gene expression control in cell-free transcription-translation systems 2
Proximity ligation assay to study protein-protein interactions of proteins on two different cells 2
Use of commercially available adhesive tapes to preserve cartilage and bone tissue integrity during cryosectioning 2
DNA barcodes from snake venom: a broadly applicable method for extraction of DNA from snake venoms 2