Nature Protocols

Nature Protocols

自然协议

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高引用文章

文章名称 引用次数
Protocol Update for large-scale genome and gene function analysis with the PANTHER classification system (v.14.0) 148
INFOGEST static in vitro simulation of gastrointestinal food digestion 132
Pathway enrichment analysis and visualization of omics data using g:Profiler, GSEA, Cytoscape and EnrichmentMap 112
Electroactive poly(vinylidene fluoride)-based structures for advanced applications 92
Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0 86
Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes 75
SHERLOCK: nucleic acid detection with CRISPR nucleases 74
Single-pot, solid-phase-enhanced sample preparation for proteomics experiments 70
Determination of hydroxyl groups in biorefinery resources via quantitative (PNMR)-P-31-N- spectroscopy 53
Targeted in situ genome-wide profiling with high efficiency for low cell numbers 53
Using DeepLabCut for 3D markerless pose estimation across species and behaviors 53
Sucrose preference test for measurement of stress-induced anhedonia in mice 49
Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization 49
Generation of human brain region-specific organoids using a miniaturized spinning bioreactor 45
Surface-wetting characterization using contact-angle measurements 44
Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography-mass spectrometry 40
Data processing, multi-omic pathway mapping, and metabolite activity analysis using XCMS Online 39
Proteome-wide identification of ubiquitin interactions using UbIA-MS 38
Generation of lung organoids from human pluripotent stem cells in vitro 35
CRISPR/Cas9 genome editing in human hematopoietic stem cells 35
Mass spectrometry based identification of MHC-bound peptides for immunopeptidomics 34
Generation and assembly of human brain region-specific three-dimensional cultures 33
High-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform 32
Design of capillary microfluidics for spinning cell-laden microfibers 31
Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry 30
Speed breeding in growth chambers and glasshouses for crop breeding and model plant research 29
Preparation of asymmetric phospholipid vesicles for use as cell membrane models 28
A cross-linking/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein-protein interactions 27
High-resolution 3D imaging of fixed and cleared organoids 26
Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing 25
Systemic AAV vectors for widespread and targeted gene delivery in rodents 25
Genome-wide mapping of endogenous G-quadruplex DNA structures by chromatin immunoprecipitation and high-throughput sequencing 24
High-resolution( 13)C metabolic flux analysis 23
Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR 23
Blood-brain-barrier organoids for investigating the permeability of CNS therapeutics 23
Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity 23
Application of anisotropic NMR parameters to the confirmation of molecular structure 22
An experimental murine model to study periodontitis 22
Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device 22
Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors 22
Production of ready-to-use few-layer graphene in aqueous suspensions 21
A nanostructure platform for live-cell manipulation of membrane curvature 21
Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip 21
Fit-free analysis of fluorescence lifetime imaging data using the phasor approach 21
Mitigating head motion artifact in functional connectivity MRI 21
Efficient and robust proteome-wide approaches for cross-linking mass spectrometry 21
Development, application and computational analysis of high-dimensional fluorescent antibody panels for single-cell flow cytometry 20
Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 &ITin vitro&IT transcripts or ribonucleoproteins 20
CRISPR-Cas9-mediated genome editing in apple and grapevine 20
Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy 19