Protein Expression And Purification

Protein Expression And Purification

蛋白质表达和纯化

  • 4区 中科院分区
  • Q4 JCR分区

高引用文章

文章名称 引用次数
Expression, purification and characterization of a recombinant antimicrobial peptide Hispidalin in Pichia pastoris 10
Isolation, purification and characterization of a novel solvent stable lipase from Pseudomonas reinekei 8
A brief practical review of size exclusion chromatography: Rules of thumb, limitations, and troubleshooting 8
Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H 8
Expression of a thermo- and alkali-philic fungal laccase in Pichia pastoris and its application 7
Cloning, expression and characterization of an endo-acting bifunctional alginate lyase of marine bacterium Wenyingzhuangia fucanilytica 7
High-level expression and characterization of a stereoselective lipase from Aspergillus oryzae in Pichia pastoris 7
Adventitious viruses in insect cell lines used for recombinant protein expression 7
Production of soluble bioactive mouse leukemia inhibitory factor from Escherichia coli using MBP tag 7
Comparison of untagged and his-tagged dihydrodipicolinate synthase from the enteric pathogen Vibrio cholerae 7
Large-scale purification of functional human P-glycoprotein (ABCB1) 7
Extracellular production of the recombinant bacterial transglutaminase in Pichia pastoris 7
Selective binding, magnetic separation and purification of histidine-tagged protein using biopolymer magnetic core-shell nanoparticles 7
High-level extracellular production of Rhizopus oryzae lipase in Pichia pastoris via a strategy combining optimization of gene-copy number with co-expression of ERAD-related proteins 6
Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli 6
Production enhancement of the extracellular lipase LipA in Bacillus subtilis: Effects of expression system and Sec pathway components 6
High-level expression and enrichment of norovirus virus-like particles in plants using modified geminiviral vectors 6
Characterization and overexpression of a glycosyl hydrolase family 16 beta-agarase YM01-1 from marine bacterium Catenovulum agarivorans YMO1(T) 6
Expression and characterization of recombinant L-asparaginase from Pseudomonas fluorescens 6
High level expression and purification of recombinant human serum albumin in Pichia pastoris 5
Heterologous expression of Trametes versicolor laccase in Saccharomyces cerevisiae 5
Rapid purification of bacteriophage endolysin TSPphg and its exogenous treatment could act as an alternative bacterial cell disruption method 5
Gene dosage and coexpression with endoplasmic reticulum secretion-associated factors improved the secretory expression of alpha-galactosidase 5
Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry 5
Enhancing recovery of recombinant hepatitis B surface antigen in lab-scale and large-scale anion-exchange chromatography by optimizing the conductivity of buffers 4
Expression of soluble, glycosylated and correctly folded dengue virus NS1 protein in Pichia pastoris 4
Cloning, overexpression, and purification of glucose-6-phosphate dehydrogenase of Pseudomonas aeruginosa 4
A two-step process for capture and purification of human basic fibroblast growth factor from E. coli homogenate: Yield versus endotoxin clearance 4
Evaluation of in vitro refolding vs cold shock expression: Production of a low yielding single chain variable fragment 4
High-level extracellular protein expression in Bacillus subtilis by optimizing strong promoters based on the transcriptome of Bacillus subtilis and Bacillus megaterium 4
Expression and purification of biologically active bovine Interferon lambda 3 (IL28B) in Pichia pastoris 4
Functional characterization of cell-free expressed K(v)1.3 channel using a voltage-sensitive fluorescent dye 4
Preparation and characterization of humanized nanobodies targeting the dimer interface of epidermal growth factor receptor (EGFR) 4
High level expression and characterization of tannase tan? using Aspergillus niger SH-2 with low-background endogenous secretory proteins as the host 4
Enhancement of the production of Bacillus naganoensis pullulanase in recombinant Bacillus subtilis by integrative expression 4
Molecular cloning and characterization of AlgL a new exo-oligoalginate lyase from Microbulbifer sp. ALW1 4
Efficient expression and isolation of recombinant human interleukin-11 (rhIL-11) in Pichia pastoris 4
An efficient constitutive expression system for Anti-CEACAM5 nanobody production in the yeast Pichia pastoris 4
A novel L-leucine 5-hydroxylase from Nostoc piscinale unravels unexpected sulfoxidation activity toward L-methionine 4
Screening novel beta-galactosidases from a sequence-based metagenome and characterization of an alkaline beta-galactosidase for the enzymatic synthesis of galactooligosaccharides 4
Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags 4
Isolation of recombinant tetrameric N-acetylated alpha-synuclein 4
Expression of a wheat beta-3-glucanase in Pichia pastoris and its inhibitory effect on fungi commonly associated with wheat kernel 4
Nanomaterial bio-activation and macromolecules functionalization: The search for reliable protocols 3
Strategies for purification of the bacteriophage HK97 small and large terminase subunits that yield pure and homogeneous samples that are functional 3
Expression and purification optimization of an N-terminal Pfs230 transmission-blocking vaccine candidate 3
Transcriptomic analysis of key genes involved in chlorogenic acid biosynthetic pathway and characterization of MaHCT from Morus alba L. 3
CodonWizard - An intuitive software tool with graphical user interface for customizable codon optimization in protein expression efforts 3
Recombinant expression and purification of a functional bacterial metallo-chaperone PbrD-fusion construct as a potential biosorbent for Pb(II) 3
Antimicrobial activity and preliminary mode of action of PlnEF expressed in Escherichia coli against Staphylococci 3