| CRISPR-Cas9 mediated targeted disruption of FAD2-2 microsomal omega-6 desaturase in soybean (Glycine max.L) |
15 |
| Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal |
15 |
| Genetic engineering of Escherichia coli to improve L-phenylalanine production |
12 |
| Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution |
11 |
| Modulation of chimeric antigen receptor surface expression by a small molecule switch |
10 |
| Production of high titer of citric acid from inulin |
10 |
| Enhancing methane production using anaerobic co-digestion of waste activated sludge with combined fruit waste and cheese whey |
9 |
| FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing |
9 |
| Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification |
8 |
| Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system |
8 |
| MicroRNA-218 promotes early chondrogenesis of mesenchymal stem cells and inhibits later chondrocyte maturation |
7 |
| A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system |
7 |
| Mucosal delivery of Lactococcus lactis carrying an anti-TNF scFv expression vector ameliorates experimental colitis in mice |
6 |
| Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing |
6 |
| Engineering a vitamin B-12 high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti |
6 |
| Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding |
5 |
| Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus |
5 |
| Optimized DNA electroporation for primary human T cell engineering |
5 |
| Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies |
5 |
| gRNA validation for wheat genome editing with the CRISPR-Cas9 system |
5 |
| Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application |
5 |
| A new prokaryotic expression vector for the expression of antimicrobial peptide abaecin using SUMO fusion tag |
5 |
| Successful production of genome-edited rats by the rGONAD method |
5 |
| Antiplasmodial and trypanocidal activity of violacein and deoxyviolacein produced from synthetic operons |
5 |
| Characterization of a novel thermostable and xylose-tolerant GH 39 beta-xylosidase from Dictyoglomus thermophilum |
5 |
| Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products |
5 |
| Genome-wide response on phytosterol in 9-hydroxyandrostenedione-producing strain of Mycobacterium sp. VKM Ac-1817D |
5 |
| Phenotype-specific recombinant haptoglobin polymers co-expressed with C1r-like protein as optimized hemoglobin-binding therapeutics |
4 |
| Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics |
4 |
| Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex |
4 |
| Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research |
4 |
| Tuning the performance of CAR T cell immunotherapies |
4 |
| Development of a vitrification method for preserving human myoblast cell sheets for myocardial regeneration therapy |
4 |
| Novel hemostatic biomolecules based on elastin-like polypeptides and the self-assembling peptide RADA-16 |
4 |
| Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803 |
4 |
| Surface patches on recombinant erythropoietin predict protein solubility: engineering proteins to minimise aggregation |
4 |
| FGF23(C-tail) improves diabetic nephropathy by attenuating renal fibrosis and inflammation |
4 |
| Expression of Rift Valley fever virus N-protein in Nicotiana benthamiana for use as a diagnostic antigen |
4 |
| Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model |
4 |
| Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein |
4 |
| Formulation and evaluation of norcanthridin nanoemulsions against the Plutella xylostella (Lepidotera: Plutellidae) |
4 |
| A paratransgenic strategy to block transmission of Xylella fastidiosa from the glassy-winged sharpshooter Homalodisca vitripennis |
4 |
| Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus |
4 |
| VARSCOT: variant-aware detection and scoring enables sensitive and personalized off-target detection for CRISPR-Cas9 |
4 |
| Metabolic flux analysis for metabolome data validation of naturally xylose-fermenting yeasts |
4 |
| Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector |
3 |
| Purification and biochemical characterization of a novel thermostable protease from the oyster mushroom Pleurotus sajor-caju strain CTM10057 with industrial interest |
3 |
| A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss |
3 |
| Preparation of a novel monoclonal antibody against caprine interleukin-17A and its applications in immunofluorescence and immunohistochemistry assays |
3 |
| Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos |
3 |
