| HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation |
27 |
| In Vivo Assimilation of One-Carbon via a Synthetic Reductive Glycine Pathway in Escherichia coli |
27 |
| High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli |
27 |
| Optimizing Oleaginous Yeast Cell Factories for Flavonoids and Hydroxylated Flavonoids Biosynthesis |
27 |
| Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii |
22 |
| An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum tricornutum |
21 |
| Biological Engineered Living Materials: Growing Functional Materials with Genetically Programmable Properties |
21 |
| Combining 26s rDNA and the Cre-IoxP System for Iterative Gene Integration and Efficient Marker Curation in Yarrowia lipolytica |
21 |
| Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry |
19 |
| Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria |
18 |
| 5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger |
18 |
| A Post-translational Metabolic Switch Enables Complete Decoupling of Bacterial Growth from Biopolymer Production in Engineered Escherichia coli |
18 |
| Gene-Mediated Chemical Communication in Synthetic Protocell Communities |
17 |
| Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins |
17 |
| Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens |
17 |
| One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells |
17 |
| Redirecting Metabolic Flux via Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in Escherichia coli |
17 |
| A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System |
17 |
| Lessons from Two Design-Build-Test-Learn Cycles of Dodecanol Production in Escherichia coli Aided by Machine Learning |
16 |
| Quantification of Interlaboratory Cell-Free Protein Synthesis Variability |
16 |
| Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2 |
16 |
| Machine Learning of Designed Translational Control Allows Predictive Pathway Optimization in Escherichia coli |
16 |
| Establishing a Cell-Free Vibrio natriegens Expression System |
15 |
| Promoter Engineering for Enhanced P(3HB-co-4HB) Production by Halomonas bluephagenesis |
15 |
| Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779 |
15 |
| Decorating the Outer Surface of Microbially Produced Protein Nanowires with Peptides |
14 |
| Engineered Reciprocal Chromosome Translocations Drive High Threshold, Reversible Population Replacement in Drosophila |
14 |
| Highly Effective and Low-Cost MicroRNA Detection with CRISPR-Cas9 |
14 |
| Rewiring Calcium Signaling for Precise Transcriptional Reprogramming |
14 |
| Identification of Novel Cryptic Multifunctional Antimicrobial Peptides from the Human Stomach Enabled by a Computational-Experimental Platform |
14 |
| Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii |
14 |
| Systematic Analysis of Bottlenecks in a Multibranched and Multilevel Regulated Pathway: The Molecular Fundamentals of L-Methionine Biosynthesis in Escherichia coli |
14 |
| Rational Design of Mini-Cas9 for Transcriptional Activation |
14 |
| Extracellular Electron Transfer by Shewanella oneidensis Controls Palladium Nanoparticle Phenotype |
13 |
| Highly Multiplexed CRISPRi Repression of Respiratory Functions Enhances Mitochondrial Localized Ethyl Acetate Biosynthesis in Kluyveromyces marxianus |
13 |
| Highly Active C-8-AcyI-ACP Thioesterase Variant Isolated by a Synthetic Selection Strategy |
13 |
| Development of Transcription Factor-Based Designer Macrolide Biosensors for Metabolic Engineering and Synthetic Biology |
13 |
| Establishing an Artificial Pathway for Efficient Biosynthesis of Hydroxytyrosol |
13 |
| A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp PCC 6803 |
13 |
| SynBioHub: A Standards-Enabled Design Repository for Synthetic Biology |
13 |
| An Orthogonal and pH-Tunable Sensor-Selector for Muconic Acid Biosynthesis in Yeast |
13 |
| Orthogonal Engineering of Biosynthetic Pathway for Efficient Production of Limonene in Saccharomyces cerevisiae |
13 |
| An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism |
13 |
| A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells |
12 |
| Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution |
12 |
| A Genetically Encoded Biosensor for Monitoring Isoprene Production in Engineered Escherichia coli |
12 |
| Modular Pathway Engineering of Bacillus subtilis To Promote De Novo Biosynthesis of Menaquinone-7 |
12 |
| Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis |
12 |
| An Adaptive Synthetic Cell Based on Mechanosensing, Biosensing, and Inducible Gene Circuits |
12 |
| Automated Design of Diverse Stand-Alone Riboswitches |
12 |
